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Int J Mol Sci 24:4079

Ni-NTA agarose beads were added to the supernatant and incubated for 1 h at 4 C

Based on the data, the authors suggest that Legionella specifically manipulates the host by differentially regulating its metabolism, upregulating activities that produce nutrients suitable for the pathogen and downregulating the host-specific metabolism. This rather general statement is in line with a more in depth biochemical study (Wieland et al., 2005) showing that expression of the amino acids transporter SLC1A5 (specific for neutral amino acids) is highly induced in the human monocyte cell line MM6 upon infection by L

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